Discussion :

[Mattilab]

Dear all,

i'm trying to analyze microscope images with Matlab.

-> What i would like:
Starting from an image, like img_di_partenza (attached, not the original resolution), i would like to filter it and highlight only the fibers.

-> What i managed to achieve
- First thing, i do is bring the grayscale image with rgb2gray.
- Increase the contrast with imadjust
- Finally I have set ‘manual threshold’.
I get more or less something like img_pos_elabora image.
N.B. the two images uploaded are not the same, they are an example.

-> Problems
- The result has a high 'signal to noise ratio'.
- I have many images and therefore I would like to automate everything (I would like to skip the step of the manual threshold).
Can someone help me?

[mach1974]

You should try the PCA with imagej to retrieve all vertices

[Mattilab]

You should try the PCA with imagej to retrieve all vertices

I tried using same imageJ plugins, but i don't know what is PCA.
What is PCA ?

TY

[mach1974]

Imagej can provide you the source .
It consists in
firstable : convert the image in grey level
Then the system will consider the image like a matrix and calculate the inverse of image
The asset goes back to highlight the vertices